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Pol II-DSIF-SPT6-PAF1c-TFIIS complex with rewrapped nucleosome
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7unc
Q-score: 0.344
Filipovski M, Soffers JHM, Vos SM, Farnung L
Science (2022) 376 pp. 1313-1316 [ DOI: doi:10.1126/science.abo3851 Pubmed: 35709268 ]
- Rna (5 kDa, RNA from synthetic construct)
- Wdr61 (33 kDa, Protein from Homo sapiens)
- Rna polymerase-associated protein ctr9 homolog (134 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from pig)
- Zinc ion (65 Da, Ligand)
- Rna polymerase ii-associated factor 1 homolog (60 kDa, Protein from Homo sapiens)
- Non-template dna (64 kDa, DNA from synthetic construct)
- Template dna (66 kDa, DNA from synthetic construct)
- Transcription elongation factor spt6 (199 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from pig)
- Rna polymerase-associated protein rtf1 homolog (80 kDa, Protein from Homo sapiens)
- Rpb11 (13 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit beta (142 kDa, Protein from pig)
- Rpb9 (14 kDa, Protein from Sus scrofa)
- Pol ii-dsif-spt6-paf1c-tfiis complex with rewrapped nucleosome (Complex)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Rna polymerase ii subunit k (7 kDa, Protein from pig)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from pig)
- Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from pig)
- Rna polymerase-associated protein leo1 (75 kDa, Protein from Homo sapiens)
- Parafibromin (60 kDa, Protein from Homo sapiens)
- Rpb8 (17 kDa, Protein from Sus scrofa)
- Transcription elongation factor a protein 1 (34 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Rpol4c domain-containing protein (20 kDa, Protein from pig)
- Dna-directed rna polymerase subunit (219 kDa, Protein from pig)
- Histone h2a (14 kDa, Protein from Xenopus laevis)
- Rpb10 (7 kDa, Protein from Sus scrofa)
Pol II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex (stalled at +38)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7und
Q-score: 0.216
Filipovski M, Soffers JHM, Vos SM, Farnung L
Science (2022) 376 pp. 1313-1316 [ DOI: doi:10.1126/science.abo3851 Pubmed: 35709268 ]
- Pol ii-dsif-spt6-paf1c-tfiis complex (stalled at +38) (Complex)
- Wdr61 (33 kDa, Protein from Homo sapiens)
- Rna polymerase-associated protein ctr9 homolog (134 kDa, Protein from Homo sapiens)
- Transcription elongation factor spt4 (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit e (24 kDa, Protein from pig)
- Zinc ion (65 Da, Ligand)
- Rna polymerase ii-associated factor 1 homolog (60 kDa, Protein from Homo sapiens)
- Non-template dna (64 kDa, DNA from synthetic construct)
- Template dna (66 kDa, DNA from synthetic construct)
- Transcription elongation factor spt6 (199 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from pig)
- Rna polymerase-associated protein rtf1 homolog (80 kDa, Protein from Homo sapiens)
- Rpb11 (13 kDa, Protein from Sus scrofa)
- Dna-directed rna polymerase subunit beta (142 kDa, Protein from pig)
- Rpb9 (14 kDa, Protein from Sus scrofa)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Rna polymerase ii subunit k (7 kDa, Protein from pig)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from pig)
- Transcription elongation factor spt5 (121 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from pig)
- Rna polymerase-associated protein leo1 (75 kDa, Protein from Homo sapiens)
- Parafibromin (60 kDa, Protein from Homo sapiens)
- Rpb8 (17 kDa, Protein from Sus scrofa)
- Transcription elongation factor a protein 1 (34 kDa, Protein from Homo sapiens)
- Rna (5 kDa, RNA from synthetic construct)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Rpol4c domain-containing protein (20 kDa, Protein from pig)
- Dna-directed rna polymerase subunit (219 kDa, Protein from pig)
- Histone h2a (14 kDa, Protein from Xenopus laevis)
- Rpb10 (7 kDa, Protein from Sus scrofa)